教授

郭占云

发布时间:2019-05-08  

姓        名:郭占云

学         位:博士

导师情况:博士生导师

研究领域:生物化学

研究方向:蛋白质与多肽

E-mailzhan-yun.guo@tongji.edu.cn     

              guozy2002@yahoo.com.cn

联系电话:021-6598-8634; 135-8574-5920

通讯地址:上海市四平路1239号,同济大学生命科学与技术学院蛋白质研究所,医学楼辅楼303房间

个人简介:

    郭占云,博士,研究员,博士生导师。1972年生,河北滦南人。1996年毕业于吉林大学分子生物学系,获学士学位。同年免试进入中国科学院上海生物化学研究所攻读博士学位,在冯佑民研究员实验室从事胰岛素超家族多肽研究,20018月获生物化学与分子生物学博士学位。2001-2003年在中国科学院生物化学与细胞生物学研究所工作,历任助理研究员和副研究员。20039月到美国Dartmouth College医学院从事博士后研究,主要研究胆固醇酯合酶1ACAT1)的结构与功能。2006年底回国到同济大学生命科学与技术学院蛋白质研究所工作,主要从事蛋白质多肽激素与G蛋白偶联受体(GPCR)相互作用研究。以通讯作者或第一作者发表SCI论文60余篇,累计影响因子超过200,被引用1200余次。已主持国家自然科学基金面上项目6项,国家重大新药创制科技重大专项1项,国家重点基础研究发展计划课题1项。现任中国生物化学与分子生物学会理事、上海市生物化学与分子生物学学会理事。

主要研究方向: 

1.多肽激素与GPCR相互作用的机制研究及新型激动剂、拮抗剂的设计开发;

2.从G蛋白偶联受体中筛选、鉴定孤儿多肽的受体;
3.多肽激素与受体相互作用研究新方法的开发;

4.植物中新型蛋白连接酶的筛选、鉴定及开发利用

发表论文:

1.Zhang CH, Shao XX, Wang XB, Shou LL, Liu YL, Xu ZG, Guo ZY*. (2022) Development of a general bioluminescent activity assay for peptide ligases. FEBS J. published online, doi: 10.1111/febs.16416.

2.Li HZ, Shou LL, Shao XX, Li N, Liu YL, Xu ZG, Guo ZY*. (2021) LEAP2 has antagonized the ghrelin receptor GHSR1a since its emergence in ancient fish. Amino Acids 53: 939-949.

3.Li HZ, Shao XX, Shou LL, Li N, Liu YL, Xu ZG, Guo ZY*. (2021) Unusual orthologs shed new light on the binding mechanism of ghrelin to its receptor GHSR1a. Arch. Biochem. Biophys. 704: 108872.

4.Li HZ, Shou LL, Shao XX, Liu YL, Xu ZG, Guo ZY* (2020) Identifying key residues and key interactions for the binding of LEAP2 to receptor GHSR1a. Biochem. J. 477: 3199-3217.

5.Li HZ, Li N, Shao XX, Liu YL, Xu ZG, Guo ZY*. (2020) Hydrophobic interactions of relaxin family peptide receptor 3 with ligands identified using a NanoBiT-based binding assay. Biochimie 177: 117-126.

6.Wang JH, Li HZ, Shao XX, Nie WH, Liu YL, Xu ZG, Guo ZY* (2019) Identifying the binding mechanism of LEAP2 to receptor GHSR1a. FEBS J. 286: 1332-1345.

7.Wang JH, Nie WH, Shao XX, Li HZ, Hu MJ, Liu YL, Xu ZG, Guo ZY* (2019) Exploring electrostatic interactions of relaxin family peptide receptor 3 and 4 with ligands using a NanoBiT-based binding assay. Biochim. Biophys. Acta Biomembr. 1861: 776-786.

8. Wang JH, Shao XX, Hu MJ, Liu YL, Xu ZG, Guo ZY* (2019) Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5. Amino Acids 51: 619-626.

9.Wang JH, Hu MJ, Zhang L, Shao XX, Lv CH, Liu YL, Xu ZG, Guo ZY* (2018) Exploring receptor selectivity of the chimeric relaxin family peptide R3/I5 by incorporating unnatural amino acids. Biochimie 154: 77-85.

10.Hu MJ, Shao XX, Li HZ, Nie WH, Wang JH, Liu YL, Xu ZG, Guo ZY* (2018) Development of a novel ligand binding assay for relaxin family peptide receptor 3 and 4 using NanoLuc complementation. Amino Acids 50: 1111-1119.

11.Wang JH, Hu MJ, Shao XX, Wei D, Liu YL, Xu ZG, Guo ZY* (2018) Cholesterol modulates the binding properties of human relaxin family peptide receptor 3 with its ligands. Arch. Biochem. Biophys. 646: 24-30.

12. Hu MJ, Wang JH, Shao XX, Liu YL, Xu ZG, Guo ZY* (2018) Overexpression of relaxin family peptide receptor 3 in Escherichia coli and characterization of its ligand binding properties. Process Biochem. 69: 131-135. 

13.Wang JH, Shao XX, Hu MJ, Wei D, Nie WH, Liu YL, Xu ZG, Guo ZY* (2017) Rapid preparation of bioluminescent tracers for relaxin family peptides using sortase-catalysed ligation. Amino Acids 49: 1611-1617.

14.Hu MJ, Wei D, Shao XX, Wang JH, Liu YL, Xu ZG, Guo ZY* (2017) Interaction mechanism of insulin-like peptide 5 with relaxin family peptide receptor 4. Arch. Biochem. Biophys. 619: 27-34.

15.Wang JH, Shao XX, Hu MJ, Wei D, Liu YL, Xu ZG, Guo ZY* (2017) A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands. Amino Acids 49: 895-903.

16.Wei D, Hu MJ, Shao XX, Wang JH, Nie WH, Liu YL, Xu ZG, Guo ZY* (2017) Development of a selective agonist for relaxin family peptide receptor 3. Sci. Rep. 7: 3230.

17.Liu Y, Zhang L, Shao XX, Hu MJ, Liu YL, Xu ZG, Guo ZY* (2016) A negatively charged transmembrane aspartate residue controls activation of the relaxin-3 receptor RXFP3. Arch. Biochem. Biophys. 604: 113-120.

18.Song G, Shao XX, Wu QP, Xu ZG, Liu YL*, Guo ZY* (2016) Novel Bioluminescent Binding Assays for Ligand–Receptor Interaction Studies of the Fibroblast Growth Factor Family. PloS One 11: e0159263.

19.Hu MJ, Shao XX, Wang JH, Wei D, Guo YQ, Liu YL, Xu ZG, Guo ZY* (2016) Mechanism for insulin-like peptide 5 distinguishing the homologous relaxin family peptide receptor 3 and 4. Sci. Rep. 6: 29648.

20.Hu MJ, Shao XX, Wang JH, Wei D, Liu YL, Xu ZG, Guo ZY* (2016) Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding. Amino Acids 48: 2227-2236.

21. Liu YL, Guo ZY* (2016) Novel bioluminescent binding assays for interaction studies of protein peptide hormones with their receptors. Amino Acids 48: 1151-1160.

22.Wu QP, Zhang L, Shao XX, Wang JH, Gao Y, Xu ZG, Liu YL*, Guo ZY* (2016) Application of the novel bioluminescent ligand–receptor binding assay to relaxin-RXFP1 system for interaction studies. Amino Acids 48: 1099-1107.

23.Song G, Wu QP, Xu T, Liu YL, Xu ZG, Zhang SF, Guo ZY* (2015) Quick preparation of nanoluciferase-based tracers for novel bioluminescent receptor-binding assays of protein hormones: using erythropoietin as a model. J. Photochem. Photobiol. B 153: 311-316.

24.Liu Y, Shao XX, Zhang L, Song G, Liu YL, Xu ZG, Guo ZY* (2015) Novel bioluminescent receptor-binding assays for peptide hormones: using ghrelin as a model. Amino Acids 47: 2237-2243.

25.Ji BJ, Song G, Zhang Z, Guo ZY* (2015) Efficient overexpression of human interleukin-6 in Escherichia coli using nanoluciferase as a fusion partner. Process Biochem. 50: 1618-1622.

26.Guo YQ, Wu QP, Shao XX, Shen T, Liu YL, Xu ZG, Guo ZY* (2015) Secretory overexpression and isotopic labeling of the chimeric relaxin family peptide R3/I5 in Pichia pastoris. Amino Acids 47: 1117-1125.

27.Liu Y, Song G, Shao XX, Liu YL, Guo ZY* (2015) Quantitative measurement of cell membrane receptor internalization by the nanoluciferase reporter: Using the G protein-coupled receptor RXFP3 as a model. Biochim. Biophys. Acta Biomembr. 1848: 688-694.

28.He SX, Song G, Shi JP, Guo YQ, Guo ZY* (2014) Nanoluciferase as a novel quantitative protein fusion tag: Application for overexpression and bioluminescent receptor-binding assays of human leukemia inhibitory factor. Biochimie 106: 140-148.

29.Wang XY, Guo YQ, Shao XX, Liu YL, Xu ZG, Guo ZY* (2014) Identification of important residues of insulin-like peptide 5 and its receptor RXFP4 for ligand-receptor interactions. Arch. Biochem. Biophys. 558: 127-132.

30.Wang XY, Guo YQ, Zhang WJ, Shao XX, Liu YL, Xu ZG, Guo ZY* (2014) The electrostatic interactions of relaxin-3 with receptor RXFP3 and the influence of its B-chain C-terminal conformation. FEBS J. 281: 2927-2936.

31.Zhang WJ, Wang XY, Guo YQ, Luo X, Gao XJ, Shao XX, Liu YL, Xu ZG, Guo ZY* (2014) The highly conserved negatively charged Glu141 and Asp145 of the G-protein-coupled receptor RXFP3 interact with the highly conserved positively charged arginine residues of relaxin-3. Amino Acids 46: 1393-1402.

32.Zhang L, Song G, Xu T, Wu QP, Shao XX, Liu YL, Xu ZG, Guo ZY* (2013) A novel ultrasensitive bioluminescent receptor-binding assay of INSL3 through chemical conjugation with nanoluciferase. Biochimie 95: 2454-2459.

33.Zhang WJ, Jiang Q, Wang XY, Song G, Shao XX, Guo ZY* (2013) A convenient method for europium-labeling of a recombinant chimeric relaxin family peptide R3/I5 for receptor-binding assays J. Pept. Sci. 19: 350-354.

34.Song G, Jiang Q, Xu T, Liu YL*, Xu ZG*, Guo ZY* (2013) A convenient luminescence assay of ferroportin internalization to study its interaction with hepcidin. FEBS J. 280: 1773-1781.

35.Luo X, Jiang Q, Song G, Liu YL*, Xu ZG*, Guo ZY* (2012) Efficient oxidative folding and site-specific labeling of human hepcidin to study its interaction with receptor ferroportin. FEBS J. 279: 3166-3175.

36.Zhang WJ, Gao XJ, Liu YL, Shao XX, Guo ZY* (2012) Design, recombinant preparation and europium-labeling of a fully active easily-labeled INSL3 analogue for receptor-binding assays. Process Biochem. 47: 1856-1860.

37.Zhang WJ, Luo X, Song G, Wang XY, Shao XX, Guo ZY* (2012) Design, recombinant expression and convenient A-chain N-terminal europium-labelling of a fully active human relaxin-3 analogue. FEBS J. 279: 1505-1512.

38.Zhang WJ, Luo X, Liu YL, Shao XX, Wade JD*, Bathgate RA*, Guo ZY* (2012) Site-specific DOTA/europium-labeling of recombinant human relaxin-3 for receptor-ligand interaction studies. Amino Acids 43: 983-992.

39.Chen GW, Luo X, Liu YL, Jiang Q, Qian XM, Guo ZY* (2011) R171H missense mutation of INSL6 in a patient with spermatogenic failure. Eur. J. Med. Genet. 54: e455-e457.

40.Luo X, Huang CH, Shao XX, Guo ZY* (2011) Design, recombinant expression and in vitro maturation of human insulin-like peptide 6 and a biotin-labeled analogue. Process Biochem. 46: 1243-1247.

41.Luo X, Liu YL, Layfield S, Shao XX, Bathgate RA*, Wade JD*, Guo ZY* (2010) A simple approach for the preparation of mature human relaxin-3. Peptides 31: 2083-2088.

42.Luo X, Bathgate RA, Zhang WJ, Liu YL, Shao XX, Wade JD*, Guo ZY* (2010)  Design and recombinant expression of insulin-like peptide 5 precursors and the preparation of mature human INSL5. Amino Acids 39: 1343-1352.

43.Wu X, Shao X, Guo ZY*, Chi CW* (2010) Identification of neuropeptide Y-like conopeptides from the venom of Conus betulinus. Acta Biochim. Biophys. Sin. 42: 502-505.

44.Wu XC, Zhou M, Peng C, Shao XX, Guo ZY*, Chi CW* (2010) Novel conopeptides in a form of disulfide-crosslinked dimer. Peptides 31: 1001-1006.

45.Wang ZQ, Zhou ZM, Guo ZY*, Chi CW* (2010) Snapshot of the interaction between HIV envelope glycoprotein 120 and protein disulfide isomerase. Acta Biochim. Biophys. Sin. 42: 358-362.

46.Luo X, Bathgate RA, Liu YL, Shao XX, Wade JD*, Guo ZY* (2009) Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3. FEBS J. 276: 5203-5211.

47.Yuan DD, Liu L, Shao XX, Peng C, Chi CW*, Guo ZY* (2009) New conotoxins define the novel I3-superfamily. Peptides 30: 861-865.

48.Liu YL, Jiang S, Ke ZM, Wu HS, Chi CW, Guo ZY* (2009) Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production. Carbohyd. Res. 344: 815-819.

49.Yuan DD, Liu L, Shao XX, Peng C, Chi CW*, Guo ZY* (2008) Isolation and cloning of a conotoxin with a novel cysteine pattern from Conus caracteristicus. Peptides 29: 1521-1525.

50.Qian J, Guo ZY*, Chi CW* (2008) Cloning and isolation of a conus cysteine-rich protein homologous to Tex31 but without proteolytic activity. Acta Biochim. Biophys. Sin. 40: 174-181.

51.Guo ZY#, Qiao ZS#, Feng YM (2008) The in vitro oxidative folding of the insulin superfamily. Antioxid. Redox Signal. 10: 127-139.

52.Guo ZY, Chang CCY, Chang TY (2007) Functionality of the seventh and eighth transmembrane domains of acyl-coenzyme A:cholesterol acyltransferase 1. Biochemistry 46: 10063-10071.

53.Wang ZQ, Han YH, Shao XX, Chi CW*, Guo ZY* (2007) Molecular cloning, expression, and characterization of protein disulfide isomerase from Conus marmoreus. FEBS J. 274: 4778-4787.

54.Guo ZY, Lin S, Henien JA, Chang CY, Chang TY (2005) The active site His-460 of human acyl-coenzyme A: cholesterol acyltransferase 1 resides in a hitherto undisclosed transmembrane domain. J. Biol. Chem. 280: 37814-37826.

55.Guo ZY, Chang CY, Lu X, Chen J, Li BL, Chang TY (2005) The disulfide linkage and the free sulfhydryl accessibility of acyl-coenzyme A:cholesterol acyltransferase 1 as studied by using mPEG5000-maleimide. Biochemistry 44: 6537-6546.

56.Guo ZY, Zhang Z, Jia XY, Tang YH, Feng YM (2005) Mutational analysis of the absolutely conserved B8Gly: consequence on foldability and activity of insulin. Acta Biochim. Biophys. Sin. 37: 673-679. 

57.Guo ZY, Jia XY, Feng YM (2004) Replacement of the interchain disulfide-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin. Biol. Chem. 385: 1171-1175.

58.Guo ZY, Wang S, Tang YH, Feng YM (2004) Mutagenesis of the three conserved valine residues: consequence on the foldability of insulin. Biochim. Biophys. Acta 1699: 103-109.

59. Jia XY#, Guo ZY#, Wang Y, Xu Y, Duan SS, Feng YM (2003) Peptide models of four possible insulin folding intermediates with two disulfides. Protein Sci. 12: 2412-2419.

60.Wang S#, Guo ZY#, Shen L, Feng YM (2003) Refolding of the amphioxus insulin-like peptide: implications of a bifurcating evolution of the different folding behaviors of insulin and insulin-like growth factor 1. Biochemistry 42: 9687-9693.

61.Guo ZY, Tang YH, Wang S, Feng YM (2003) Contribution of the absolutely conserved B8Gly to the foldability of insulin. Biol. Chem. 384: 805-809.

62.Yan H#, Guo ZY#, Gong YW, Xi D, Feng YM (2003) A peptide model of insulin folding intermediate with one disulfide. Protein Sci. 12: 768-775.

63.Guo ZY, Shen L, Gu W, Wu AZ, Ma JG, Feng YM (2002) In vitro evolution of amphioxus insulin-like peptide to mammalian insulin. Biochemistry 41: 10603-10607.

64.Guo ZY, Shen L, Feng YM (2002) The different energetic state of the intra A-chain/domain disulfide of insulin and insulin-like growth factor 1 is mainly controlled by their B-chain/domain. Biochemistry 41: 10585-10592.

65.Guo ZY, Shen L, Feng YM (2002) The different folding behavior of insulin and insulin-like growth factor 1 is mainly controlled by their B-chain/domain. Biochemistry 41: 1556-1567. 

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