Modeling human lung development and disease using human pluripotent stem cells    

 

Sarah Xuelian Huang, MD, PhD, 

Associate Research Scientist, 

Department of Medicine, Columbia University Medical Center, New York, New York, USA.    

 

简介：

 

Directed differentiation of human pluripotent stem cells (hPSCs), either embryonic stem cells (ESCs) or induced PSCs (iPSCs), into specific organ cell types provides a unique platform for human disease modeling and drug discovery, as well as the advancement of regenerative medicine. Generating lung tissue and other anterior foregut endoderm derivatives from hPSCs is challenging, and has lagged behind progress on directed differentiation into many other tissues types.

We have established, based on developmental paradigms, a highly efficient method for directed differentiation of hPSCs into lung and airway epithelial cells. Long-term differentiation in vivo and in vitro yielded cells expressing markers of basal, goblet, Clara, ciliated, type I and type II alveolar epithelial (AT I and II) cells and were particularly enriched in distal ATII cells capable of surfactant protein-B (SP-B) uptake and release. This technology may lead to the development of novel approaches for tissue or organ replacement therapy in the distance future, and has immediate applications in modeling human lung development and disease. First, we applied this technology to model genetic predisposition to severe influenza infection. Second, since our cells generated are particularly enriched in distal ATII cells, we successfully modeled human distal lung development using a three-dimensional (3D) lung hydrogel culture system. Embedded cells formed structures compatible with those from saccular/alveolar stage of developing lung.

时间: 5月7日上午10：00-11：30

地点：同济大学医学楼1102室