Gene Isoform Analysis by Third Generation

Topic: Gene Isoform Analysis by Third Generation

Speaker: Kinfai AU, Ph.D. Department of Internal Medicine and Biostatistics，University of Iowa, USA

Host: ZHANG Chao

Time: 10:00AM- 11:30AM, 13th MAR, 2015

Venue: Room 1102, Medical Building

Introduction of the speaker:

1. Research focus

RNA-Seq and Third Generation Sequencing We are interested in methodology development of Third Generation Sequencing (TGS) (especially for PacBio and Oxford Nanopore sequencing). Au lab is working on both hybrid sequencing (Second Generation Sequencing (SGS) + TGS) and TGS-alone methodology research. Our research interests include but not limited to alternative splicing, isoform construction, gene fusion and quantitative analysis of transcriptome.

 Stem Cell Transcriptome Analysis Au lab is applying the hybrid sequencing method on ESC, iPSC and preimplantation embryo, to deeply study the transcriptome differences between stem cells.

Employment and Education  2013.12-present Assistant Professor, Carver College of Medicine, University of Iowa, USA  2009.10-2013.6 Postdoctoral scholar, Department of Statistics, Stanford University, USA  2007.9-2009.6 M.S. in Statistics, Stanford University, USA  2004.10-2009.9 Ph.D. in Structural Biology, University of Oxford, UK  2000.9-2004.7 B.S. in Biological Sciences, Tsinghua University, China

2. Selected publications

Au, Kin Fai* et al., The transcriptome of human pluripotent stem cells. Curr Opin Genet Dev. 2014

 Au, Kin Fai et al., Characterization of the human ESC transcriptome by hybrid sequencing. Proc. Natl. Acad. Sci. USA, 2013

Au, Kin Fai et al., Improving PacBio long read accuracy by short read alignment. PLoS ONE, 2012

Tan, M.H. #, Au, Kin Fai# et al., RNA sequencing reveals diverse and dynamic repertoire of the Xenopus tropicalis transcriptome over development. Genome Research, 2012

Au, Kin Fai et al., Detection of splice junctions from paired-end RNA-seq data by SpliceMap. Nucleic Acids Research, 2010