报告题目：Zebrafish As a Discovery Platform for Molecular Mechanisms of Type 2 Diabetes  

报 告 人：陈文标，博士，副教授

Vanderbilt University，U.S.A.

主 持 人：张 超

时    间：2016年11月9日 周三 上午10:00-11:30

地    点：医学大楼1102室

Research Background

    The pancreatic islet employs several mechanisms to maintain glucose homeostasis. In addition to the acute change of glucagon and insulin secretion from a and b cells in response to low and high blood glucose, respectively, insufficient action of glucagon and insulin also triggers compensatory hyperplasia of the cognate cells. Defects in b-cell compensation and b-cell maintenance result in diabetes, and compensatory a-cell hyperplasia can lead to endocrine tumors. I will present our recent work on using zebrafish as a model to understand the molecular mechanisms of overnutrition-induced b-cell genesis, glucagon deficiency-induced a-cell hyperplasia, and chronic overnutrition-induced b-cell death.

Employment and Education

• 2013-           Associate Professor, Vanderbilt University Medical School, Nashville, TN, USA

• 2008-2013   Assistant Professor, Vanderbilt University Medical School, Nashville, TN, USA

• 2001-2008   Assistant Scientist, Vollum Institute, OHSU, Portland, OR, USA

• 1997-2001   Postdoctoral Associate, MIT, Cambridge, MA, USA

• 1993-1997   PhD., OHSU, Portland, OR, USA

• 1991-1993   MS, Washington State University, Pullman, WA, USA

Selected Publications

1. Chen W, Kelly MA, Opitz-Araya X, Thomas RE, Low MJ, and Cone RD. (1997). Exocrine glands dysfunction in MC5-R-deficient mice: Evidence for coordinated regulation of exocrine gland function by melanocortin peptides. Cell 91,789-798.

2. Chen W, Burgess S, Hopkins N.  (2001). Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. Development. 128(12):2385-96.

3. Maddison LA, Chen W. (2012) Nutrient Excess Stimulates b-Cell Neogenesis in Zebrafish. Diabetes. 61:2517-24

4. Ni TT, Lu J, Zhu M, Maddison LA, Huskey LC, Boyd K, Ju B, Hesselson D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W. (2012) Conditional control of gene function by an invertible gene trap in zebrafish. PNAS, 109:15389-94.

5. Jao LE, Wente S, Chen W. (2013) Efficient multiplex biallelic zebrafish genome editing using the CRISPR nuclease. PNAS 110(34):13904-9.