房健民

姓         名:房健民  

学         位:博士

职         称:教授

导师情况:博士生导师

研究领域:细胞和分子生物学

研究方向:单克隆抗体和蛋白质工程
                 新药肿瘤生物学
                 干细胞生物学

E-mail:jfang@tongji.edu.cn

联系电话

通讯地址:上海市四平路1239号,同济大学生命科学与技术学院(200092)

房健民教授简介:

     1982年浙江农业大学学士,1986在南京农业大学硕士。1993至1997年在加拿大Dalhousie大学生物系攻读生物学博士学位,1997-2000年 在美国哈佛大学医学院从事博士后研究。从2000年至2009年,在美国历任Cell Genesys研究公司研究员,高级研究员,新药开发项目负责人;Panorama研究所高级研究员,抗体工程研究部主任。2009年始任同济大学生命科 学与技术学院教授,博士生导师。

     主要研究兴趣在抗肿瘤 和自身免疫疾病的生物药物开发,肿瘤血管新生的分子调控,干细胞的分化等。发表文章的刊物包括Nature Biotechnology, PNAS, Cancer Research, Molecular Therapy, Developmental Biology等。有多项已授权或公开的发明专利。曾获中国国家科技进步奖二等奖,加拿大Killam 学者奖,加拿大NSERC博士后奖等。

房健民教授实验室欢迎校内外学生和青年研究者报考硕士或博士研究生, 或进行博士后研究。

 

代表性论文(#一作,*通讯):

  1. Bingyu Li, Lijun Xu, Chenyu Pi, Yanxin Yin, Kun Xie, Fei Tao, Renhao Li, Hua Gu*, and Jianmin Fang* CD89-mediated recruitment of macrophages via a bispecific antibody enhances anti-tumor efficacy Oncoimmunology 2017 Oct 12;7(1):e1380142  https://doi.org/10.1080/2162402X.2017.1380142
  2. You Li, Xiao Z. Shen, Liang Li, Tuantuan V. Zhao,Kenneth E. Bernstein, Alan K. Johnson,; Patrick Lyden, Jianmin Fang*, Peng Shi* Brain Transforming Growth Factor- Resists Hypertension Via Regulating Microglial Activation. Stroke. 2017 Sep;48(9):2557-2564. 
  3. Kaiming Chen, Dong Li, Hongwen Li, Bing Li, Jie Li, LeiHuang, Renhao Li, Xiaoqing Xu, Lingxiao Jiang, Cizhong Jiang, Hua Gu, Jianmin Fang*, Genetic analysis of heterogeneous sub-clones in recombinant Chinese hamster ovary cells. Applied Microbiology and Biotechnology 2017 Jul;101(14):5785-5797. 
  4. Bingyu Li, Lijun Xu, Fei Tao, Kun Xie, Zhiqiang Wu, You L1, Jie Li, Kaiming Chen, Chenyu Pi, Andrew Mendelsohn, James W. Larrick, Hua Gu*, Jianmin Fang* Simultaneous exposure to FcγR and FcαR on monocytes and macrophages enhances antitumor activity in vivo. Oncotarget. 2017 Jun 13;8(24):39356-39366.
  5. Jingxing Zhang, Qiong Cai , Ming Jiang , Yigang Liu , Hua Gu , Jia Guo , Hui Sun , Jianmin Fang*, Lingjing Jin*, Mesencephalic astrocyte-derived neurotrophic factor alleviated 6-OHDA-induced cell damage via ROS-AMPK/mTOR mediated autophagic inhibition Experimental Gerontology 89 (2017 Mar) 45–56
  6. Wenwei Hu*, Yang Yang*, Longzhen Zhang, Jianxin Yin, Jingwei Huang, Lei Huang, Hua Gu, Gening Jiang* & Jianmin Fang* Post surgery circulating free tumor DNA is a predictive biomarker for relapse of lung cancer Cancer Med. 2017 May;6(5):962-974. doi: 10.1002/cam4.980. Epub 2017 Apr 5.
  7. Lijun Xu, Bingyu Li, Mengwen Huang, Kun Xie, DongLi, You Li, Hua Gu*, Jianmin Fang* Critical role of Kupffer cell CD89 expression in experimental IgA Nephropathy. PLos ONE 2016 Jul 20;11(7):e0159426.
  8. Dong Li, Kun Xie, Longzhen Zhang, Xuejing Yao, Hongwen Li, Qiaoyu Xu, XinWang, Jing Jiang, Jianmin Fang* Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effects. Cancer Letters 377 (2016) 164–173
  9. Dong Li,Jiejun Shi, Yanhua Du, Kaiming Chen, Zhenping Liu, Bing Li, Jie Li, Fei Tao, Hua Gu, Cizhong Jiang*, Jianmin Fang*  Profiling Analysis of Histone Modifications and Gene Expression in Lewis Lung Carcinoma Murine Cells Resistant to Anti-VEGF Treatment PLoS ONE 2016 Jun 11(6): e0158214. doi:10.1371/journal.pone.0158214
  10. Huang J, Chen C, Gu H, Li C, Fu X, Jiang M, Sun H, Xu J, Fang J, Jin L. Mesencephalic astrocyte-derived neurotrophic factor reduces cell apoptosis via upregulating GRP78 in SH-SY5Y cells. Cell Biol Int. 2016 May 4. doi: 10.1002/cbin.10621.
  11. Hongwen Li, Kaiming Chen, Zhe Wang, Dong Li, Jiannan Lin, Chao Yu, Fang Yu, Xin Wang, Lei Huang, Cizhong Jiang, Hua Gu and Fang J*. Genetic analysis of clonal stability of Chinese hamster ovary cells for recombinant protein production. Mol BioSys., 12(1), pp 102-109, 2016.Jan
  12. Li H, Yu C, Jiang J, Huang C, Yao X, Xu Q, Yu F, Lou L, Fang J* An anti-HER2 antibody conjugated with monomethyl auristatin E is highly effective in HER2-positive human gastric cancer. Cancer Biol Ther. 2016 Apr 2;17(4):346-54. doi: 10.1080/15384047.2016.1139248. Epub 2016 Feb 6.
  13. Jing Jiang, Lihou Dong, LeiWang, LingWang, Jing Zhang, Fang Chen, Xiuli Zhang, Min Huang, Shenjun Li, Weiwei Ma, Qiaoyu Xu, Changjiang Huang, Jianmin Fang*, Chunhua Wang* HER2-targeted antibody drug conjugates for ovarian cancer therapy. European Journal of Pharmaceutical Sciences 93 (2016) 274–286
  14. Xia Chen, Qian Zhao, Yong Hou, Ji Jiang, Wen Zhong, Wenxiang Wang, Xuejing Yao, Lin Li, Jianmin Fang*, Fengchun Zhang* and Pei Hu* Pharmacokinetics, pharmacodynamics, short term efficacy and safety of RCT-18, a novel BLyS/APRIL fusion protein, in patients with rheumatoid arthritis Br J Clin Pharmacol (2016) 82 41–52
  15. Qian Zhao∗, Xia Chen∗, Yong Hou, Ji Jiang,Wen Zhong,Xuejing Yao, WenxiangWang, Lin L, Jianmin Fang, Fengchun Zhang, and Pei Hu* Pharmacokinetics, Pharmacodynamics, Safety, and Clinical Activity of Multiple Doses of RCT-18 in Chinese Patients With Systemic Lupus Erythematosus. The Journal of Clinical Pharmacology 2016, 56(8) 948–959
  16. Wei Zhu∗, Kun Xie, Yuanjian Xu, Le Wang, Kaiming Chen, Longzhen Zhang, Jianmin Fang∗ CRISPR/Cas9 produces anti-hepatitis B virus effect in hepatoma cellsand transgenic mouse  Virus Research 217 (2016) 125–132
  17. Wei Zhu, Jie Li, Yigang Liu, Kun Xie, Le Wang & Jianmin Fang* Mesencephalic astrocyte-derived neurotrophic factor attenuates inflammatory responses in lipopolysaccharide-induced neural stem cells by regulating NF-κB and phosphorylation of p38-MAPKs pathways Immunopharmacology and  Immunotoxicology 2016VOL. 38, NO. 3, 205–213
  18. Xuejing Yao,Jing Jiang,Xin Wang,Changjiang Huang,Dong Li,Kuan Xie Qiaoyu Xu,Hongwen Li,Zhuanglin Li,Liguang Lou,Jianmin Fang*  A novel humanized anti-HER2 antibody conjugated with MMAE exerts potent anti-tumor activity. Breast Cancer Res Treat 2015 Aug;153(1):123-33. doi: 10.1007/s10549-015-3503-3. 
  19. Xia Chen, Yong Hou, Ji Jiang, Qian Zhao, Wen Zhong, Wenxiang Wang, Xuejing Yao, Lin Li, Jianmin Fang*, Fengchun Zhang*, Pei Hu* Pharmacokinetics, Pharmacodynamics, and Tolerability of Single Ascending Doses of RCT-18 in Chinese Patients with Rheumatoid Arthritis Clin Pharmacokinet (2014) 53:1033–1044 DOI 10.1007/s40262-014-0175-9
  20. D Li, X Wei, K Xie, K Chen, J Li and J Fang*, A novel decoy receptor fusion protein for FGF-2 potently inhibits tumor growth. British Journal of Cancer (2014) 111, 68–77 | doi: 10.1038/bjc.2014.282
  21. Li D, Xie K Xie, Ding G, Li J, Chen K, Li H, Qian J, Jiang C, Fang J*. Tumor resistance to anti-VEGF therapy through up-regulation of VEGF-C expression. Cancer Letter. Cancer Letters 346 (2014) 45–52
  22. Zhou S, Yang Y, Yang Y, Tao H, Li D, Zhang J, Jiang G, Fang J*. Combination Therapy of VEGF-Trap and Gemcitabine Results in Improved Anti-Tumor Efficacy in a Mouse Lung Cancer Model. PLoS One.8 (7),2013 
  23. Simmons AD, Moskalenko M, Creson J, Fang J, Yi S, Vanroey MJ, Allison JP, Jooss K. Local secretion of anti-CTLA-4 enhances the therapeutic efficacy of a cancer immunotherapy with reduced evidence of systemic autoimmunity. Cancer Immunol Immunother. 57:1263-70. 2008.
  24. Fang J, Yi S, Harding TC, Tu GH, VanRoey M, Jooss K. An antibody delivery system for regulated expression of monoclonal antibody at therapeutic level in vivo. Molecular Therapy  15:1153-1159. 2007.
  25. Harper J, Yan L, Loureiro RM, Wu I, Fang J, D’Amore PA, Moses MA. Repression of vascular endothelial growth factor expression by the zinc finger transcription factor ZNF24. Cancer Research. 67:8736-8741. 2007.
  26. Fang J, Qian JJ, Yi S, Harding TC, Tu GH, VanRoey M, Jooss K. Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotechnology 23:584-590. 2005.
  27. Fang J. and Jooss K. Rapid generation of high-level antibodies in vitro and in vivo. Discovery Medicine 5:367-370. 2005.
  28. Marler JJ, Fishman SJ, Kilroy SM, Fang J, Upton J, Mulliken JB, Burrows PE, Zurakowski D, Folkman J, Moses MA. Increased Expression of Urinary Matrix Metalloproteinases Parallels The Extent and Activity of Vascular Anomalies. Pediatrics 116:38-45. 2005.
  29. Fang J, Yan L, Shing Y, Moses MA. HIF-1alpha-mediated up-regulation of vascular endothelial growth factor, independent of basic fibroblast growth factor, is important in the switch to the angiogenic phenotype during early tumorigenesis. Cancer Research 61:5731-5735. 2001.
  30. Fang J., Shing Y., Wiederschain D., Yan L., Butterfield C., Jackson G., Harper J., Tamvakopoulos G., Moses M.A. Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in a tumor model. Proceedings of the National Academy of Sciences, USA (PNAS) 97:3884-3889. 2000.
  31. Fang J. and Hall B.K. N-CAM is not required for initiation of secondary chondrogenesis: The role of N-CAM in skeletal condensation and differentiation. International Journal of Developmental Biology 43: 335-342. 1999.
  32. Fang J. and Hall B.K.  Chondrogenic cell differentiation from membrane bone periostea. Anatomy and Embryology 196: 349-362. 1997.
  33. Fang J. and Hall B.K.  In vitro differentiation potential of the periosteal cells from a membrane bone, the quadratojugal of the embryonic chick. Developmental Biology 180: 701-712. 1996.
  34. Fang J. and Hall B.K.  Differential expression of neural cell adhesion molecule (NCAM) during osteogenesis and secondary chondrogenesis in the embryonic chick. International Journal of Developmental Biology 39: 519-528. 1995.

 

承担项目:

  1. 上海市科委创新基础项目(18JC1414400),CD89介导的新型双特异性抗体肿瘤免疫治疗研究 2018/06-2021/05,80万元,主持
  2. 国家重点基础研究发展计划(973计划),2015CB553706,炎-癌生物信号交互调控癌进展及阻抑治疗分子机制2015/01-2019/06,110万元,主持
  3. 国家自然科学基金面上项目,31470896,通过IgA Fc 受体(FcαRI)介导的ADCC 效应提高单克隆抗体抗肿瘤作用及机制研究,2015/01-2018 /12,80万元,主持
  4. 高等学校创新能力提升计划(2011计划)生物治疗协同创新中心,160万
  5. 国家自然科学基金面上项目,31270987,影响抗体药物在重组CHO细胞株中稳定高效表达的关键因子的研究,2013/01-2016/12,80万元,主持
  6. 国家自然科学基金面上项目,81371403,调控表达MANF 治疗帕金森病的作用及机制研究,2013/01-2016/12,80万元,参与
  7. 上海市科学技术委员会科技支撑项目(12431901000)新型抗体融合蛋白和骨形态发生蛋白的成药性研究,2012/09-2015/09,30万元,主持
  8. 上海市浦江人才计划,肿瘤血管新生靶向治疗中耐药性形成的分子机制,2010/07-2012/06,20万元,主持
  9. 上海市科学技术委员会-应用基础研究,10140900400,小鼠肺腺癌生长及肿瘤新生血管抑制的疗效研究,2010/05-2012/09,20万元,主持

 

专著/专利:

  1. 房健民,优化的TACI-Fc 融合蛋白, 中国、美国、俄罗斯、韩国、日本等, ZL200710111162.2 
  2. 房健民,优化的TACI-Fc 融合蛋白用于制备治疗类风湿性关节炎药物的应用,中国、美国、俄罗斯、韩国、日本等,ZL201110021339.6 
  3. 房健民 ,优化的TACI-Fc 融合蛋白用于制备治疗系统性红斑狼疮药物的应用,中国、美国、俄罗斯、韩国、日本等,ZL 201110021344.7 
  4. 房健民,李栋,拮抗血管新生诱导因子的融合蛋白及其用途,中国、美国、俄罗斯、韩国、日本等,ZL201110131029.X 
  5. 房健民,李栋,FGFR-Fc 融合蛋白及其用途,中国、美国、俄罗斯、韩国、日本等, ZL 201110132218.9 
  6. 房健民,腺相关病毒介导可调控表达的MANF 载体的构建,中国, 201310091541.5 
  7. 房健民,周爽,杨洋,李栋,可用于评估药物治疗效果的Luc-LLC 肿瘤模型及其建立与应用,中国,201210444846.5 
  8. 房健民,郭佳,李晨,一种重组TAT-MANF 融合蛋白及其应用, 中国,: 201310156113.6 
  9. 房健民. 稳定表达荧光素酶的A549 裸鼠模型及其建立与应用,中国,201210444335.3 
  10. 房健民, 周爽, 杨洋,李栋. 整体可视化人肺腺癌H1650 裸鼠模型及其建立与应用 , 中国,201210445078.5 
  11. 房健民. 人源化抗HER2 单克隆抗体与抗体-药物偶联及其用途。 已申请。 
  12. Fang, Jianmin, Jooss, Karin, Qian, Jing-Jing. Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof. 美国,7,485,291. 
  13. Fang,Jianmin, . Angiogenesis-inhibiting chimeric protein and the use, 美国7,750,138 
  14. Fang, Jianmin, Jooss, Karin, Simmons, Andrew, Qian, Jing-Jing. Compositions and ,methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site. 美国,7709224 
  15. Fang; Jianmin, Jooss; Karin, Qian; Jing Jing. AAV vector compositions and methods for enhanced expression of immunoglobulins using the same. 美国,7,714,119 
  16. Fang; Jianmin, Jooss; Karin, Simmons; Andrew, Qian; Jing-Jing. Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site. 美国, 7,662,623 
  17. Fang, Jianmin, Jooss, Karin, Qian, Jing-Jing. Compositions and methods for enhanced expression of immunoglobulins from a single vector using a peptide cleavage site. 2009.3.3, 美国,7,498,024 
  18. Fang, Jianmin; Jooss, Karin; Simmons, Andrew; Farson, Debbie; Methods to express recombinant proteins from lentiviral vectors. 美国, 7,632,509

 

获奖情况:

  1. 中国侨界贡献奖 2016.7.19
  2. 房健民 2015中国产学研合作创新奖 2015
  3. Fang J. The NSERC Fellowship, National Sciences and Engineering Research Council of Canada (NSERC), 2000 
  4. Fang J. The Izaak Walton Killam Memorial Scholarship, Dalhousie University, Halifax, Nova Scotia, Canada, 1994, 1995, 1996 
  5. 夏德全、吴婷婷、杨弘、房健民、董在杰。 奥利亚罗非鱼遗传特性和抗寒相关因子研究, 中国国家科技进步奖二等奖, 1995 

 

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