林古法

姓        名:林古法

学       位:博士

导师情况:博导

研究领域:器官再生, 干细胞生物学

研究方向:1、创伤愈合及断肢/断指再生的细胞与分子生物学机制
                      2、器官前体细胞诱导分化机理

电子邮箱lingufa@tongji.edu.cn; lingufa@gmail.co

联系电话:021-65983272

通讯地址:上海市四平路1239号 同济大学生命科学与技术学院(200092)

个人简介

  1996年毕业于复旦大学,医学学士;1996年7月-1999年7月任青岛大学医学院附属医院住院医师;2004年中科院上海生命科学研究院发育生物学专业毕业,博士;2004年9月-2007年8月任英国巴斯大学(University of Bath)博士后研究员(Research Officer);2007年10月-2011年6月任美国明尼苏达大学Research Associate; 2011年6月起任明尼苏达大学遗传细胞生物学与发育系(GCD)Assistant Professor; 2013年10月起任同济大学生命科学与技术学院教授。


研究领域(具体内容)

  

    本实验室研究器官再生的细胞与分子生物学机理并探讨诱导哺乳动物器官再生的途径。我们新近在国际上首次通过移植含有胚胎肢芽前体细胞和生长因子组合的凝胶纤维蛋白骨架(fibrin scaffold)建立了诱导断肢再生的技术方法。该发现经发表后即获得了强烈的关注(被F1000等推荐)。我们前期对胚胎肢芽前体细胞是否可以用于促进小鼠断趾再生进行了探索,结果提示胚胎肢芽前体细胞移植是促进断指/断肢再生的有效途径。 目前实验室致力于利用诱导多能干细胞(iPSC)探讨多能前体细胞移植在肢体损伤处置中的应用前景。

  我们实验室已建立了多个小鼠和人iPS细胞系。这些人iPS细胞系均通过episomal 系统建立,具有极好的分化潜能,无外源基因整合。目前实验室已建立一套不依赖于滋养层细胞无动物源的培养系统,对从人iPS细胞获得组织前体细胞奠定了重要基础。

 

论文论著

Book Chapters:

  1. Chen Y, Lin G*. (2014). Xenopus model for regeneration. Xenopus Development, Edited by Kubiak J & Kloc M. Willy-Blackwell Publishing. *corresponding author
  2. Leng H, Lin G* and Chen Y*. (2013). Cellular Origins of Amphibian Regeneration. Progenitor Cells: Biology, Characterization and Potential Clinical Applications, Edited by Horton P. and Lawrence B. Nova Publishers, New York (book chapter). *corresponding author

 

Peer-reviewed publications:

  1. Zhang M, Chen Y, Xu H, Yang L, Yuan F, Li L, Xu Y, Chen Y*, Zhang C*, and Lin G* (2018). Melanocortin receptor 4 (Mc4r) signaling regulates vertebrate limb regeneration. Dev Cell.2018.46: 397-409. *corresponding author
  2. Zhang M, Yang L, Yuan F, Chen Y, and Lin G*. Dicer inactivation stimulates limb regeneration ability in Xenopus laevis. Wound Repair and Regeneration. 2018. 26:46-53. *corresponding author
  3. Chen Y, Xu H, Lin G*. Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discovery 2017 (3), 17046; doi:10.1038/celldisc.2017.46. *corresponding author
  4. Lin H, Zhu X, Chen G, Song L, Gao L, Khand A, Chen Y, Lin G* and Tao Q*. KDM3A-mediated demethylation of histone H3 lysine 9 facilitates the chromatin binding of Neurog2 during neurogenesis. Development 2017, 144:3674-3685. *corresponding author
  5. Pai, V.P., Lemire, J.M., Chen, Y., Lin, G., and Levin, M. (2015). Local and long-range endogenous resting potential gradients antagonistically regulate apoptosis and proliferation in the embryonic CNS. Int J Dev Biol. 2015, 59:327-340.
  6. Pai V, Lemire JM, Pare JF, Lin G, Chen Y and Levin M. (2015) Endogenous gradients of resting potential instructively pattern neural tissue via Notch signalling. J. Neurosci. 35: 4366–4385.
  7. Shi X, Jai R, Zirbes K, Gong W, Lin G, Kyba M, Thomson J, Koyano-Nakagawa N and Garry D. (2014). Cooperative interaction of Etv2 and Gata2 regulates the development of endothelial and hematopoietic lineages Dev. Biol. 389, 208–218..
  8. Lin G* *, Chen Y.* and Slack JMW*. (2013). Imparting regenerative capacity to limbs by progenitor cell transplantation. Dev. Cell 24: 41-51  * equal contributor.   * corresponding author. (F1000prime recommended: http://f1000.com/prime/717971589?bd=1&ui=22057)
  9. Lin G*, Chen Y. and Slack JMW. (2012). Transgenic analysis of signaling pathways required for Xenopus tadpole spinal cord and muscle regeneration. Anat. Rec. 295: 1532-1540. * corresponding author.
  10. Chen Y, Lin G, Chen Y, Fok A and Slack JMW. (2012). Micro-computed tomography for visualizing limb skeletal regeneration in young Xenopus frogs. Anat. Rec. 295: 1562-1565.
  11. Lin G and Slack JMW. (2008). Requirement for Wnt and FGF signaling in Xenopus tadpole tail regeneration. Dev Biol. 316: 323-335.
  12. Slack JMW, Lin G and Chen Y. (2008). The Xenopus tadpole: a new model for regeneration research. Cell Mol Life Sci. 65: 54-63.
  13. Lin G, Chen Y and Slack JMW. (2007). Regeneration of neural crest derivatives in the Xenopus tadpole tail. BMC Dev Biol 7, 56.
  14. Chen Y, Lin, G. and Slack, J. M. (2006). Control of muscle regeneration in the Xenopus tadpole tail by Pax7. Development 133, 2303-13.
  15. Lin G, Geng X, Chen Y, Qu B, Wang F, Hu R and Ding X. (2003). T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos. Dev Dyn 226, 51-8.
  16. Wu K, Weng Z, Lin G, Wu X, Li F and Zhang Y. (2005). The effect of breast cancer specific gene 1 in the prognosis of breast cancer. Chinese Journal of Surgery. 43:294-7 (in Chinese).
  17. Wu K, Weng Z, Lin G, Yin M, Qian H, Zhang Y, Ding X. (2004). Significance of breast cancer specific gene 1 expression in breast cancer. Shanghai Medical Journal. 27:193-5  (in Chinese).
  18. Lin G, Geng X, Chen Y, Qu B, Wang F, Hu R and Ding X. (2003). T-box binding site mediates the dorsal activation of myf-5 in Xenopus gastrula embryos. Dev Dyn 226, 51-8.
  19. Geng X, Xiao L, Lin G, Hu R, Wang JH, Rupp RA and Ding X. (2003). Lef/Tcf-dependent Wnt/beta-catenin signaling during Xenopus axis specification. FEBS Lett 547, 1-6.
  20. Chen Y#, Lin G#, Hu R, Chen Y and Ding X. (2003). Activin/Nodal signals mediate the ventral expression of myf-5 in Xenopus gastrula embryos. Biochem Biophys Res Commun 310, 121-7. #equal contributor.
  21. Wu K, Weng Z, Tao Q, Lin G, Wu X, Qian H, Zhang Y, Ding X, Jiang Y, Shi YE. (2003). Stage-specific expression of breast cancer-specific gene gamma-synuclein. Cancer Epidemiol Biomarkers Prev. 12: 920-5.
 

实验室欢迎有兴趣的本科生、研究生及博士后加盟(email: lingufa@tongji.edu.cn)。

 

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